Directed DNA deamination by AID/APOBEC3 in immunity
نویسندگان
چکیده
منابع مشابه
Directed DNA deamination by AID/APOBEC3 in immunity
Several hundred papers in the past seven years have established two new mechanisms of immunity, both of which are dependent upon proteins that deaminate cytosines to uracils within singlestranded DNA. Activation-induced deaminase (AID) deaminates C -> U to implement the diversification of vertebrate antibody genes and mammalian APOBEC3 proteins, such as human APOBEC3F and APOBEC3G, deaminate C ...
متن کاملDNA Deamination Mediates Innate Immunity to Retroviral Infection
CEM15/APOBEC3G is a cellular protein required for resistance to infection by virion infectivity factor (Vif)-deficient human immunodeficiency virus (HIV). Here, using a murine leukemia virus (MLV)-based system, we provide evidence that CEM15/APOBEC3G is a DNA deaminase that is incorporated into virions during viral production and subsequently triggers massive deamination of deoxycytidine to deo...
متن کاملDNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B
APOBEC (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like) is a family of enzymes that deaminates cytosine (C) to uracil (U) on nucleic acid. APOBEC3B (A3B) functions in innate immunity against intrinsic and invading retroelements and viruses. A3B can also induce genomic DNA mutations to cause cancer. A3B contains two cytosine deaminase domains (CD1, CD2), and there are conflictin...
متن کاملElectrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.
متن کاملCytidine Deamination of Retroviral DNA by Diverse APOBEC Proteins
The human cytidine deaminase APOBEC3G edits both nascent human immunodeficiency virus (HIV) and murine leukemia virus (MLV) reverse transcripts, resulting in loss of infectivity. The HIV Vif protein is able to protect both viruses from this innate restriction to infection. Here, we demonstrate that a number of other APOBEC family members from both humans and rodents can mediate anti-HIV effects...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Biology
سال: 2006
ISSN: 0960-9822
DOI: 10.1016/j.cub.2006.02.035